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验证数据展示

  • WB analysis of mouse cerebellum using 21505-1-AP (same clone as 21505-1-PBS)

    WB analysis of mouse cerebellum using 21505-1-AP (same clone as 21505-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

    IP experiment of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

    IP result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 21505-1-AP (same clone as 21505-1-PBS)

    IF Staining of A549 using 21505-1-AP (same clone as 21505-1-PBS)

    Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

    WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

    WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

  • WB analysis using 21505-1-AP (same clone as 21505-1-PBS)

    WB analysis using 21505-1-AP (same clone as 21505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 21505-1-AP (same clone as 21505-1-PBS)

    IHC staining of human kidney using 21505-1-AP (same clone as 21505-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Arp5/ACTR5 antibody included
  • Featured Product
  • KD/KO Validated

Arp5/ACTR5 Polyclonal antibody, PBS Only

Arp5/ACTR5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21505-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ACTR5, ARP5, Actin related protein 5, Actin-related protein 5, hARP5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Arp5/ACTR5 Ready-To-Use IHC Kit. Arp5/ACTR5 primary antibody included.

产品信息

21505-1-PBS targets Arp5/ACTR5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15976

Product name: Recombinant human ACTR5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 258-607 aa of BC038402

Sequence: EELHKWRCPDYYENNVHKMQLPFSSKLLGSTLTSEEKQERLQQQLRRLQELNARRREEKLQLDQERLDRLLYVQELLEDGQMDQFHKALIELNMDSPEELQSYIQKLSIAVEQAKQKILQAEVNLEVDVVDSKPETPDLEQLEPSLEDVESMNDFDPLFSEETPGVEKPVTTVQPVFNLAAYHQLFVGTERIRAPEIIFQPSLIGEEQAGIAETLQYILDRYPKDVQEMLVQNVFLTGGNTMYPGMKARMEKELLEMRPFRSSFQVQLASNPVLDAWYGARDWALNHLDDNEVWITRKEYEEKGGEYLKEHCASNIYVPIRLPKQASRSSDAQASSKGSAAGGGGAGEQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ARP5 actin-related protein 5 homolog (yeast)
别名 ACTR5, ARP5, Actin related protein 5, Actin-related protein 5, hARP5
计算分子量 607 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC038402
基因名称 ARP5/ACTR5
Gene ID (NCBI) 79913
RRIDAB_10733470
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H9F9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

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