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验证数据展示

  • WB analysis using 31454-1-AP

    WB analysis using 31454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31454-1-AP

    WB analysis using 31454-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 31454-1-AP

    IHC staining of rat cerebellum using 31454-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat cerebellum using 31454-1-AP

    IHC staining of rat cerebellum using 31454-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 31454-1-AP

    IF Staining of HepG2 using 31454-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apoe antibody (31454-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Apoe Polyclonal antibody

Apoe Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31454-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

AI255918, apolipoprotein E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-251 cells, BV-2 cells, C2C12 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse cerebellum tissue, rat brain tissue, pig cerebellum tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31454-1-AP targets Apoe in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Apoe fusion protein Eg1093 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Apolipoprotein E
别名 AI255918, apolipoprotein E
计算分子量36kDa
观测分子量31~36 kDa
GenBank蛋白编号NM_001305819.1
基因名称 Apoe
Gene ID (NCBI) 11816
RRIDAB_3669993
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP08226
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).

实验方案

Product Specific Protocols
WB protocol for Apoe antibody 31454-1-APDownload protocol
IHC protocol for Apoe antibody 31454-1-APDownload protocol
IF protocol for Apoe antibody 31454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols