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验证数据展示

  • IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

    IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

    IHC staining of human colon cancer using 11497-1-AP (same clone as 11497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 11497-1-AP (same clone as 11497-1-PBS)

    IHC staining of mouse brain using 11497-1-AP (same clone as 11497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse placenta using 11497-1-AP (same clone as 11497-1-PBS)

    IHC staining of mouse placenta using 11497-1-AP (same clone as 11497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

    IHC staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11497-1-AP (same clone as 11497-1-PBS)

    IF Staining of HepG2 using 11497-1-AP (same clone as 11497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

    IF Staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

    IF Staining of human placenta using 11497-1-AP (same clone as 11497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11497-1-AP (same clone as 11497-1-PBS)

    IF Staining of mouse brain using 11497-1-AP (same clone as 11497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11497-1-PBS in a different storage buffer formulation.

Apelin Polyclonal antibody, PBS Only

Apelin Polyclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 11497-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IF-P, Indirect ELISA

别名

APLN, APEL, APJ endogenous ligand

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11497-1-PBS targets Apelin in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2047

Product name: Recombinant human APLN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-77 aa of BC021104

Sequence: MNLRLCVQALLLLWLSLTAVCGGSLMPLPDGNGLEDGNVRHLVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMPF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apelin
别名 APLN, APEL, APJ endogenous ligand
计算分子量 77 aa, 9 kDa
GenBank蛋白编号BC021104
基因名称 Apelin
Gene ID (NCBI) 8862
RRIDAB_2877771
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULZ1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apelin, isolated from bovine stomach tissue extractsis, is recognized as the endogenous ligand of the angiotensin-like-receptor 1 (APJ), and the human orphan G-protein-coupled receptor. Apelin is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. Apelin has been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system. Apelin is also known to be involved in the maintenance of INS sensitivity and play an important role in liver disease.

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