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验证数据展示

  • WB analysis using RMX00072

    WB analysis using RMX00072

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00072 (Annexin V antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using RMX00072

    IHC staining of human placenta using RMX00072

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00072 (Annexin V antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using RMX00072

    IHC staining of human placenta using RMX00072

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00072 (Annexin V antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using RMX00072

    IF Staining of HeLa using RMX00072

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin V antibody (RMX00072) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® Annexin V Multi-Recombinant antibody

Annexin V Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00072

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Annexin A5, ANXA5, Anchorin CII, Annexin 5, Annexin-5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00072 targets Annexin V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 annexin A5
别名 Annexin A5, ANXA5, Anchorin CII, Annexin 5, Annexin-5
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC001429
基因名称 Annexin V
Gene ID (NCBI) 308
RRIDAB_3750121
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP08758
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

实验方案

Product Specific Protocols
IF protocol for Annexin V antibody RMX00072Download protocol
IHC protocol for Annexin V antibody RMX00072Download protocol
WB protocol for Annexin V antibody RMX00072Download protocol
Standard Protocols
Click here to view our Standard Protocols
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