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验证数据展示

  • WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

Annexin A4 Monoclonal antibody, PBS Only

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60288-2-PBS

产品说明书

CloneNo. 1A10B5

宿主/亚型

Mouse / IgG2c

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60288-2-PBS targets Annexin A4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0130

Product name: Recombinant human Annexin IV protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-202 aa of BC000182

Sequence: GTDEDAIISVLAYRNTAQRQEIRTAYKSTIGRDLIDDLKSELSGNFEQVIVGMMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVLVSLSAGGRDEGNYLDDALVRQDAQDLYEAGEKKWGTDEVKFLTVLCSR

种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A4
别名 35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV
计算分子量 36 kDa
观测分子量 32 kDa
GenBank蛋白编号BC000182
基因名称 Annexin A4
Gene ID (NCBI) 307
RRIDAB_2881405
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09525
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

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