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验证数据展示

  • WB analysis using 25153-1-AP

    WB analysis using 25153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25153-1-AP

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 25153-1-AP

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 40x lens).

Annexin A13 Polyclonal antibody

Annexin A13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25153-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse small intestine tissue, rat small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25153-1-AP targets Annexin A13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Annexin A13 fusion protein Ag18581 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 annexin A13
别名 ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13
计算分子量 316 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC125158
基因名称 Annexin A13
Gene ID (NCBI) 312
RRIDAB_2879928
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27216
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A13 (ANXA13) is believed to be the original founder gene of the 12-member vertebrate annexin A family, and it has acquired an intestine-specific expression associated with a highly differentiated intracellular transport function (PMID: 11961095). ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family (PMID: 28423508). Limited evidence suggests that ANXA13 may play a role in apoptotic cell-mediated immunosuppression, hypertension, and cell death recognition (PMID: 28423508). ANXA13 is highly expressed in the human colon adenocarcinoma cell line HT29.

实验方案

Product Specific Protocols
WB protocol for Annexin A13 antibody 25153-1-APDownload protocol
IHC protocol for Annexin A13 antibody 25153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel 1,4-Benzodiazepine Derivative as a Highly Selective ANXA3 Degrader for the Treatment of Triple-Negative Breast Cancer

Authors - Kunlin Liu
View Article