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Plasminogen Polyclonal antibody

Plasminogen Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 18300-1-AP

Print datasheet

Synonyms

Angiostatin, plasminogen, PLG

验证数据展示

  • WB analysis using 18300-1-AP

    WB analysis using 18300-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 18300-1-AP

    IHC staining of human liver using 18300-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 18300-1-AP

    IHC staining of human liver using 18300-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 18300-1-AP

    IF Staining of HepG2 using 18300-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18300-1-AP (Plasminogen antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

18300-1-AP targets Plasminogen in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plasminogen
Synonyms Angiostatin, plasminogen, PLG
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC060513
Gene Symbol PLG
Gene ID (NCBI) 5340
RRIDAB_2167096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

实验方案

Product Specific Protocols
WB protocol for Plasminogen antibody 18300-1-APDownload protocol
IHC protocol for Plasminogen antibody 18300-1-APDownload protocol
IF protocol for Plasminogen antibody 18300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Int Immunol

Zoobiquity experiments show the importance of the local MMP9-plasminogen axis in inflammatory bowel diseases in both dogs and patients

Authors - Takeshi Yamasaki
View Article