验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83816-1-RR targets Angiopoietin-2 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | angiopoietin 2 |
| 别名 | Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2 |
| 计算分子量 | 56KD |
| 观测分子量 | 68kDa |
| GenBank蛋白编号 | NM_007426.4 |
| 基因名称 | Angpt2 |
| Gene ID (NCBI) | 11601 |
| RRID | AB_3671402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O35608 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Angiopoietin-2 antibody 83816-1-RR | Download protocol |
| IHC protocol for Angiopoietin-2 antibody 83816-1-RR | Download protocol |
| IP protocol for Angiopoietin-2 antibody 83816-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |