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Host
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Species Reactivity
0
Species Reactivity
0
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0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83816-1-RR (same clone as 83816-1-PBS)

    WB analysis using 83816-1-RR (same clone as 83816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

    IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

    IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

    IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

    IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 83816-1-RR (same clone as 83816-1-PBS)

    IP experiment of mouse lung using 83816-1-RR (same clone as 83816-1-PBS)

    IP result of anti-Angiopoietin-2 (IP:83816-1-RR, 4ug; Detection:83816-1-RR 1:1500) with mouse lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83816-1-RR

    Affinity and Kinetic Characterization of 83816-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83816-1-RR against Mouse Angiopoietin-2 were performed. The affinity constant is below 1 pM.

Mouse Angiopoietin-2 Recombinant antibody, PBS Only

Angiopoietin-2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 83816-1-PBS

产品说明书

CloneNo. 240760D5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IP, ELISA

别名

Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83816-1-PBS targets Angiopoietin-2 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 angiopoietin 2
别名 Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2
计算分子量56KD
观测分子量 68kDa
GenBank蛋白编号NM_007426.4
基因名称 Angpt2
Gene ID (NCBI) 11601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO35608
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).