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验证数据展示

  • WB analysis of MCF-7 using 16036-1-AP (same clone as 16036-1-PBS)

    WB analysis of MCF-7 using 16036-1-AP (same clone as 16036-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IHC staining of human stomach cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AREG antibody included
  • IP experiment of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)

    IP experiment of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)

    IP result of anti-Amphiregulin (IP:16036-1-AP, 4ug; Detection:16036-1-AP 1:2000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 16036-1-AP (same clone as 16036-1-PBS)

    FC experiment of HeLa using 16036-1-AP (same clone as 16036-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Amphiregulin Polyclonal antibody, PBS Only

Amphiregulin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA
Cat No. 16036-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, FC (Intra), IP, ELISA

别名

AREG, AR, SDGF, CRDGF, Colorectum cell-derived growth factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AREG Ready-To-Use IHC Kit. AREG primary antibody included.

产品信息

16036-1-PBS targets Amphiregulin in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 Amphiregulin fusion protein Ag8907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amphiregulin
别名 AREG, AR, SDGF, CRDGF, Colorectum cell-derived growth factor
计算分子量 252 aa, 28 kDa
观测分子量 43 kDa
GenBank蛋白编号BC009799
基因名称 Amphiregulin/AREG
Gene ID (NCBI) 374
RRIDAB_2227602
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15514
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).