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验证数据展示

  • WB analysis of NIH/3T3 using 66221-1-Ig (same clone as 66221-1-PBS)

    WB analysis of NIH/3T3 using 66221-1-Ig (same clone as 66221-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66221-1-Ig (Alpha E catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 66221-1-Ig (same clone as 66221-1-PBS)

    IHC staining of human stomach using 66221-1-Ig (same clone as 66221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTNNA1 antibody included
  • IHC staining of human stomach using 66221-1-Ig (same clone as 66221-1-PBS)

    IHC staining of human stomach using 66221-1-Ig (same clone as 66221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTNNA1 antibody included
  • FC experiment of MCF-7 using 66221-1-Ig (same clone as 66221-1-PBS)

    FC experiment of MCF-7 using 66221-1-Ig (same clone as 66221-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Alpha E-Catenin (66221-1-Ig, Clone:2B7B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

Alpha E-Catenin Monoclonal antibody, PBS Only

Alpha E-Catenin Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66221-1-PBS

产品说明书

CloneNo. 2B7B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

CTNNA1, 2B7B8, alpha catenin, Alpha E catenin, CAP102

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CTNNA1 Ready-To-Use IHC Kit. CTNNA1 primary antibody included.

产品信息

66221-1-PBS targets Alpha E-Catenin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23603

Product name: Recombinant human CTNNA1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 188-536 aa of BC031262

Sequence: SEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), alpha 1, 102kDa
别名 CTNNA1, 2B7B8, alpha catenin, Alpha E catenin, CAP102
计算分子量 906 aa, 100 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC031262
基因名称 Alpha E-Catenin
Gene ID (NCBI) 1495
RRIDAB_2881612
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35221
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

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