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验证数据展示

  • WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

    FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (66895-1-Ig, Clone:1E2E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (Alpha Actinin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (Alpha Actinin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

    IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Alpha Actinin antibody (66895-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

Alpha Actinin Monoclonal antibody, PBS Only

Alpha Actinin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66895-1-PBS

产品说明书

CloneNo. 1E2E1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ACTN1, Alpha actinin 1, 1E2E1, actin, actinin, alpha 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

产品信息

66895-1-PBS targets Alpha Actinin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag28383

Product name: Recombinant human a-Actinin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 543-892 aa of BC015766

Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 actinin, alpha 1
别名 ACTN1, Alpha actinin 1, 1E2E1, actin, actinin, alpha 1
计算分子量 103 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC015766
基因名称 Alpha Actinin
Gene ID (NCBI) 87
RRIDAB_2882224
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

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