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验证数据展示

  • WB analysis of HepG2 using 67308-1-Ig

    WB analysis of HepG2 using 67308-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67308-1-Ig

    IHC staining of human liver using 67308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67308-1-Ig

    IHC staining of human liver using 67308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67308-1-Ig

    IF Staining of HepG2 using 67308-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

Alpha 2-Antiplasmin Monoclonal antibody

Alpha 2-Antiplasmin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67308-1-Ig

产品说明书

CloneNo. 3F6C3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, API, PLI, Serpin F2, SERPINF2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human plasma, THP-1 cells, K-562 cells, HuH-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67308-1-Ig targets Alpha 2-Antiplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag4095

Product name: Recombinant human SERPINF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 146-491 aa of BC031592

Sequence: HLLSRLCQDLGPGAFRLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDDLANINQWVKEATEGKIQEFLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLTQRDSFHLDEQFTVPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
别名 A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, API, PLI, Serpin F2, SERPINF2
计算分子量 491 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC031592
基因名称 Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2882569
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08697
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
IHC protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
WB protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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