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验证数据展示

  • WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)

    WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

    IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

    IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

    IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

    IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)

    WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51427-1

    Cytometric bead array sample test of MP51427-1

    Cytometric bead array sample test of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS.

  • Cytometric bead array standard curve of MP51427-1

    Cytometric bead array standard curve of MP51427-1

    Cytometric bead array standard curve of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS. Standard:Ag6779. Range: 0.781-100 ng/mL.

Aldolase C Monoclonal antibody, PBS Only (Capture)

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66120-1-PBS

产品说明书

CloneNo. 5H7A5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66120-1-PBS targets Aldolase C as part of a matched antibody pair:

MP51427-1: 66120-1-PBS capture and 66120-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6779

Product name: Recombinant human ALDOC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-364 aa of BC003613

Sequence: MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldolase C, fructose-bisphosphate
别名 ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC003613
基因名称 Aldolase C
Gene ID (NCBI) 230
RRIDAB_2881519
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09972
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).