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验证数据展示

  • IF Staining of mouse liver using 82783-6-RR

    IF Staining of mouse liver using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 82783-6-RR

    IF Staining of mouse liver using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of Human Hepatocellular cancer using 82783-6-RR

    IHC staining of Human Hepatocellular cancer using 82783-6-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82783-6-RR (Albumin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALB antibody included
  • IHC staining of human stomach using 82783-6-RR

    IHC staining of human stomach using 82783-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALB antibody included
  • IHC staining of human stomach using 82783-6-RR

    IHC staining of human stomach using 82783-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALB antibody included
  • IF Staining of HepG2 using 82783-6-RR

    IF Staining of HepG2 using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 82783-6-RR

    WB analysis using 82783-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82783-6-RR (Albumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Albumin Recombinant monoclonal antibody

Albumin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 82783-6-RR

产品说明书

CloneNo. 4K1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

ALB, 4K1, HSA, Serum albumin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma, HepG2 cells
Positive IHC detected inhuman stomach tissue, Human Hepatocellular cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ALB Ready-To-Use IHC Kit. ALB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82783-6-RR targets Albumin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9569

Product name: Recombinant human Albumin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 256-609 aa of BC034023

Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 albumin
别名 ALB, 4K1, HSA, Serum albumin
计算分子量 609 aa, 69 kDa
观测分子量66 kDa
GenBank蛋白编号BC034023
基因名称 Albumin
Gene ID (NCBI) 213
RRIDAB_3670549
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

实验方案

Product Specific Protocols
IF protocol for Albumin antibody 82783-6-RRDownload protocol
IHC protocol for Albumin antibody 82783-6-RRDownload protocol
WB protocol for Albumin antibody 82783-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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