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验证数据展示

  • WB analysis using 31340-1-AP

    WB analysis using 31340-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31340-1-AP (Albumin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 31340-1-AP

    IHC staining of rat liver using 31340-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31340-1-AP (Albumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat liver using 31340-1-AP

    IF Staining of rat liver using 31340-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using Albumin antibody (31340-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Albumin Polyclonal antibody

Albumin Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31340-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Alb, Serum albumin, rat albumin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31340-1-AP targets Albumin in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 albumin
别名 Alb, Serum albumin, rat albumin
计算分子量69 kDa
观测分子量69 kDa
基因名称 Alb
Gene ID (NCBI) 24186
RRIDAB_3669953
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02770
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Albumin is a multi-functional protein that participates in the regulation of colloid osmotic pressure, transportation of endogenous ligands and drugs, and regulation of microvascular permeability. Albumin major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Human (HSA) and rat (RSA) serum albumins share similar characteristics of binding biologically active substances.

实验方案

Product Specific Protocols
WB protocol for Albumin antibody 31340-1-APDownload protocol
IHC protocol for Albumin antibody 31340-1-APDownload protocol
IF protocol for Albumin antibody 31340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols