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验证数据展示

  • IHC staining of human lung cancer using 30532-1-AP

    IHC staining of human lung cancer using 30532-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30532-1-AP (Aggrecan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACAN antibody included
  • IHC staining of human lung cancer using 30532-1-AP

    IHC staining of human lung cancer using 30532-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30532-1-AP (Aggrecan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACAN antibody included
  • FC experiment of ATDC-5 using 30532-1-AP

    FC experiment of ATDC-5 using 30532-1-AP

    1x10^6 ATDC-5 cells were intracellularly stained with 0.25 ug Aggrecan Polyclonal Antibody (30532-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, ELISA
Cat No. 30532-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inATDC-5 cells
Planning an IHC experiment? We recommend our IHCeasy ACAN Ready-To-Use IHC Kit. ACAN primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30532-1-AP targets Aggrecan in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32723

Product name: Recombinant human ACAN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-153 aa of BC036445

Sequence: MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aggrecan
别名 ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2
计算分子量 250 kDa
GenBank蛋白编号BC036445
基因名称 ACAN
Gene ID (NCBI) 176
RRIDAB_3742348
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

实验方案

Product Specific Protocols
FC protocol for Aggrecan antibody 30532-1-APDownload protocol
IHC protocol for Aggrecan antibody 30532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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