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Adropin Polyclonal antibody

Adropin Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 25213-1-AP

Print datasheet

Synonyms

Adropin, C9orf165, energy homeostasis associated, ENHO, UNQ470

验证数据展示

  • IHC staining of human liver using 25213-1-AP

    IHC staining of human liver using 25213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 25213-1-AP

    IHC staining of human liver using 25213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 25213-1-AP

    IF Staining of SH-SY5Y using 25213-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Adropin antibody (25213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25213-1-AP targets Adropin in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human
Immunogen Adropin fusion protein Ag19526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name energy homeostasis associated
Synonyms Adropin, C9orf165, energy homeostasis associated, ENHO, UNQ470
Calculated Molecular Weight 75 aa, 8 kDa
GenBank Accession NumberBC022101
Gene Symbol ENHO
Gene ID (NCBI) 375704
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adropin is a nutritionally regulated peptide hormone that is primarily secreted by the liver and is important in maintaining metabolic and non-metabolic homeostasis. Adropin regulates glucose and fatty acid metabolism and exerts a potential protective effect on endothelial function through modulation of endothelial nitric oxide (NO) synthesis. It is associated with obesity and is involved in the prevention of insulin resistance, dyslipidemia, and impaired glucose tolerance (PMID: 22318315).

实验方案

Product Specific Protocols
IHC protocol for Adropin antibody 25213-1-APDownload protocol
IF protocol for Adropin antibody 25213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols