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验证数据展示

  • WB analysis using RMX00098

    WB analysis using RMX00098

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00098 (Adiponectin receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using RMX00098

    IHC staining of mouse liver using RMX00098

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00098 (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using RMX00098

    IF Staining of mouse liver using RMX00098

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (RMX00098) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Multi-rAb® Adiponectin receptor Multi-Recombinant antibody

Adiponectin receptor Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. RMX00098

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

缓冲液配方:  PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00098 targets Adiponectin receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 adiponectin receptor 2
别名
计算分子量 44 kDa
观测分子量45 kDa
GenBank蛋白编号BC051858
基因名称 Adiponectin receptor
Gene ID (NCBI) 79602
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDQ86V24
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Adiponectin receptor antibody RMX00098Download protocol
IHC protocol for Adiponectin receptor antibody RMX00098Download protocol
WB protocol for Adiponectin receptor antibody RMX00098Download protocol
Standard Protocols
Click here to view our Standard Protocols
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