×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using RMX00098

    WB analysis using RMX00098

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00098 (Adiponectin receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using RMX00098

    IHC staining of mouse liver using RMX00098

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00098 (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using RMX00098

    IF Staining of mouse liver using RMX00098

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (RMX00098) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Multi-rAb® Adiponectin receptor Multi-Recombinant antibody

Adiponectin receptor Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. RMX00098

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

ADIPOR2, ACDCR2, adiponectin receptor 2, Adiponectin receptor protein 2, ADIPOR1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00098 targets Adiponectin receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 adiponectin receptor 2
别名 ADIPOR2, ACDCR2, adiponectin receptor 2, Adiponectin receptor protein 2, ADIPOR1
计算分子量 44 kDa
观测分子量45 kDa
GenBank蛋白编号BC051858
基因名称 Adiponectin receptor
Gene ID (NCBI) 79602
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDQ86V24
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 12802337). The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.

实验方案

Product Specific Protocols
IF protocol for Adiponectin receptor antibody RMX00098Download protocol
IHC protocol for Adiponectin receptor antibody RMX00098Download protocol
WB protocol for Adiponectin receptor antibody RMX00098Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...