acetylated Tubulin(Lys40) Monoclonal antibody

acetylated Tubulin(Lys40) Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, Canine, pig and More (3)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7E5H8

Cat No : 66200-1-Ig

Print datasheet

Synonyms

Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, Acetylated tubulin (Lys40), acetylated Tubulin(Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, tubulin



经过测试的应用

Positive WB detected inrat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis
Positive IHC detected inmouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, mouse brain tissue
Positive ELISA detected inRecombinant protein
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

66200-1-Ig targets acetylated Tubulin(Lys40) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine, pig samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat, Canine, pig
Cited Reactivityhuman, mouse, rat, bovine, Drosophila, pig, zebrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tubulin, alpha 1a
Synonyms Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, Acetylated tubulin (Lys40), acetylated Tubulin(Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, tubulin
Calculated molecular weight 52 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberNM_006009
Gene symbol TUBA1A
UniprotQ71U36
Gene ID (NCBI) 7846
RRIDAB_2722562
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

 

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

 

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

 

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

 

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

 

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

 

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).


实验方案

Product Specific Protocols
WB protocol for acetylated Tubulin(Lys40) antibody 66200-1-IgDownload protocol
IHC protocol for acetylated Tubulin(Lys40) antibody 66200-1-IgDownload protocol
IF protocol for acetylated Tubulin(Lys40) antibody 66200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Clin Invest

Nasal ciliated cells are primary targets for SARS-CoV-2 replication in early stage of COVID-19.

Authors - Ji Hoon Ahn
WB

Biomaterials

A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions.

Authors - Ji Wang
DrosophilaWB

Dev Cell

HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.

Authors - Yan Yan
IF

Am J Hum Genet

C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia.

Authors - Mahmoud R Fassad
humanWB

Theranostics

The histone deacetylase inhibitor PCI-24781 impairs calcium influx and inhibits proliferation and metastasis in breast cancer.

Authors - Tianshu Yang
mouseIF

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang