验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, rat cerebellum tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84863-5-RR targets ACBP/DBI in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 | ACBP/DBI fusion protein Eg2462 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | diazepam binding inhibitor |
| 别名 | 242463D1, ACBP, Acyl-CoA-binding protein, DBI, Diazepam-binding inhibitor |
| 计算分子量 | 10KDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | NM_007830.4 |
| 基因名称 | Dbi |
| Gene ID (NCBI) | 13167 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P31786 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acbp/Dbi (acyl-CoA binding protein/Diazepam-binding inhibitor) is a widely expressed protein that binds long-chain fatty acyl-CoA esters and plays a role in fatty acyl-CoA transport and pool formation. Acbp/Dbi is critical to cellular proliferation, differentiation, mitochondrial functions, and autophagy. Deletion of Acbp in mouse results in sebocyte hyperplasia and sparse, matted hair with a greasy appearance (PMID: 32632162, 16902415).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACBP/DBI antibody 84863-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |