验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, BxPC-3 cells, HeLa cells, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20540-1-AP targets AXIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag14574 Product name: Recombinant human AXIN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-83 aa of BC006295 Sequence: EGETPPCQPGVGKGQVTKPMPVSSNTRRNEDGLGEPEGRASPDSPLTRWTKSLH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | axin 2 |
| 别名 | Axin-2, Axil, axin 2, Axin like protein, Axin-like protein |
| 计算分子量 | 843 aa, 94 kDa |
| 观测分子量 | 94-100 kDa |
| GenBank蛋白编号 | BC006295 |
| 基因名称 | AXIN2 |
| Gene ID (NCBI) | 8313 |
| RRID | AB_10694569 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2T1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulates Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites and can also undergo poly(ADP-ribosylation)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosylated)lated AXIN2 then gets ubiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm, and its mutation is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibody raised against the 34-amino-acid N-terminus of human AXIN2. The observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for AXIN2 antibody 20540-1-AP | Download protocol |
| WB protocol for AXIN2 antibody 20540-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin | ||
Signal Transduct Target Ther Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation. | ||
Cell Res Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress. | ||
Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. | ||
Autophagy TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer. |
