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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 68093-1-Ig (same clone as 68093-1-PBS)

    WB analysis using 68093-1-Ig (same clone as 68093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 68093-1-Ig (same clone as 68093-1-PBS)

    IHC staining of mouse stomach using 68093-1-Ig (same clone as 68093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AXIN1 antibody included
  • IHC staining of mouse stomach using 68093-1-Ig (same clone as 68093-1-PBS)

    IHC staining of mouse stomach using 68093-1-Ig (same clone as 68093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AXIN1 antibody included
  • IF Staining of A431 using 68093-1-Ig (same clone as 68093-1-PBS)

    IF Staining of A431 using 68093-1-Ig (same clone as 68093-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AXIN1 antibody (68093-1-Ig, Clone: 1C4E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 68093-1-Ig (same clone as 68093-1-PBS)

    FC experiment of A431 using 68093-1-Ig (same clone as 68093-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human AXIN1 (68093-1-Ig, Clone:1C4E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

AXIN1 Monoclonal antibody, PBS Only

AXIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68093-1-PBS

产品说明书

CloneNo. 1C4E8

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

hAxin, Axis inhibition protein 1, Axin-1, AXIN 1, AXIN

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AXIN1 Ready-To-Use IHC Kit. AXIN1 primary antibody included.

产品信息

68093-1-PBS targets AXIN1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 AXIN1 fusion protein Ag10079 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 axin 1
别名 hAxin, Axis inhibition protein 1, Axin-1, AXIN 1, AXIN
计算分子量826aa,92 kDa; 862aa,95 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC044648
基因名称 AXIN1
Gene ID (NCBI) 8312
RRIDAB_2918830
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15169
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC.