验证数据展示
经过测试的应用
| Positive WB detected in | 769-P cells, 786-O cells, Caki-1 cells, Caki-2 cells, OS-RC-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27691-1-AP targets AVPR2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag24835 Product name: Recombinant human AVPR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-83 aa of BC112181 Sequence: MLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLSIVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | arginine vasopressin receptor 2 |
| 别名 | ADHR, Antidiuretic hormone receptor, AVPR V2, DI1, DIR |
| 计算分子量 | 371 aa, 40 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC112181 |
| 基因名称 | AVPR2 |
| Gene ID (NCBI) | 554 |
| RRID | AB_3742249 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30518 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AVPR2 is a Gs-coupled vasopressin receptor essential for renal water reabsorption, acting through cAMP-PKA-dependent trafficking of aquaporin-2. Localized to principal cells of the collecting duct, AVPR2 integrates hormonal cues to maintain osmotic and fluid homeostasis. Mutations lead to X-linked nephrogenic diabetes insipidus, while rare activating variants cause SIADH. Because of its central role in renal physiology and clear clinical associations, AVPR2 is a key target in water-balance disorders and pharmacologic modulation of diuresis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AVPR2 antibody 27691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
