验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25001-1-AP targets AUTS2 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | AUTS2 fusion protein Ag21853 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | autism susceptibility candidate 2 |
| 别名 | AUTS2, FBRSL2, KIAA0442 |
| 计算分子量 | 139 kDa |
| 观测分子量 | 70 kDa, 130-140 kDa |
| GenBank蛋白编号 | BC064693 |
| 基因名称 | AUTS2 |
| Gene ID (NCBI) | 26053 |
| RRID | AB_2879840 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WXX7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AUTS2 also named as FBRSL2 or autism susceptibility candidate 2 is a 1259 amino acid protein, which belongs to the AUTS2 family. AUTS2 is highly expressed in brain, kidney and skeletal muscle. AUTS2 exists as four isoforms produced by alternative splicing events and is encoded by a gene located on human chromosome 7. The molecular weight of AUTS2 isoform is 65-70 kDa in the brain ( PMID:23332918). The functional role of AUTS2 is not well known, although some studies have identified a putative role in transcriptional regulation during neuronal development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AUTS2 antibody 25001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Neurol Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model. | ||
Mol Cell Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities. | ||
Exp Neurol Auts2 regulated autism-like behavior, glucose metabolism and oxidative stress in mice | ||
Nucleic Acids Res CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver |
