Aurora B Monoclonal antibody
Aurora B Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
IHC, WB,ELISA
Conjugate
Unconjugated
CloneNo.
3H1
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse thymus tissue |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
60098-1-Ig targets Aurora B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Aurora B fusion protein Ag7532 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | aurora kinase B |
Synonyms | AIK2, AIM 1, AIM1, ARK 2, ARK2, AurB, AURKB, Aurora B, aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, IPL1, STK 1, STK12, STK5 |
Calculated Molecular Weight | 39.2 kDa |
Observed Molecular Weight | 39-45 kDa |
GenBank Accession Number | BC013300 |
Gene Symbol | AURKB |
Gene ID (NCBI) | 9212 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96GD4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis. | ||
Hum Mol Genet Downregulation of Survivin contributes to cell-cycle arrest during postnatal cardiac development in a severe spinal muscular atrophy mouse model. | ||
PLoS One Anti-pancreatic cancer deliverables from sea: first-hand evidence on the efficacy, molecular targets and mode of action for multifarious polyphenols from five different brown-algae. | ||
FEBS J YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells |