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验证数据展示

  • IF Staining of HepG2 using 17528-1-AP

    IF Staining of HepG2 using 17528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AURKAIP1 antibody (17528-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

AURKAIP1 Polyclonal antibody

AURKAIP1 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 17528-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

28S ribosomal protein S38, mitochondrial, AIP, AKIP, AURKA interacting protein, AURKA-interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17528-1-AP targets AURKAIP1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 AURKAIP1 fusion protein Ag11444 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aurora kinase A interacting protein 1
别名 28S ribosomal protein S38, mitochondrial, AIP, AKIP, AURKA interacting protein, AURKA-interacting protein
计算分子量 199 aa, 22 kDa
GenBank蛋白编号BC062333
基因名称 AURKAIP1
Gene ID (NCBI) 54998
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AURKAIP1 (Aurora-A kinase interacting protein 1) has been identified as an Aurora-A kinase interacting protein involving in two degradation pathways of Aurora-A, including proteasome-dependent pathway and Ub-independent pathway . Ectopic expression of AURKAIP1 resulted in the down-regulation of Aurora-A protein levels and thus, AURKAIP1 could be regarded as a cancer suppressor involving Aurora-A pathway.(PMID: 38040691,PMID: 17125467)

实验方案

Product Specific Protocols
IF protocol for AURKAIP1 antibody 17528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols