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验证数据展示

  • WB analysis using 82906-1-RR (same clone as 82906-1-PBS)

    WB analysis using 82906-1-RR (same clone as 82906-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82906-1-RR (AURKA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82906-1-RR

    Affinity and Kinetic Characterization of 82906-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82906-1-RR against Human AURKA were performed. The affinity constant is below 1 pM.

AURKA Recombinant monoclonal antibody, PBS Only

AURKA Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82906-1-PBS

产品说明书

CloneNo. 230087H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Aurora A, AURK, AURA, ARK-1, AIRK1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

产品信息

82906-1-PBS targets AURKA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0362

Product name: Recombinant human AURKA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 108-337 aa of BC002499

Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aurora kinase A
别名 Aurora A, AURK, AURA, ARK-1, AIRK1
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC002499
基因名称 AURKA
Gene ID (NCBI) 6790
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14965
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

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