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验证数据展示

  • IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AURKA antibody included
  • IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AURKA antibody included
  • WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50265-1

    Cytometric bead array standard curve of MP50265-1

    Cytometric bead array standard curve of MP50265-1, AURKA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66757-2-PBS. Detection antibody: 66757-1-PBS. Standard:Ag20096. Range: 0.391-50 ng/mL.

  • Featured Product
  • KD/KO验证

AURKA Monoclonal antibody, PBS Only (Detector)

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66757-1-PBS

产品说明书

CloneNo. 1F4B10

宿主/亚型

Mouse / IgG3

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Aurora A, 1F4B10, AIRK1, ARK-1, AURA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

产品信息

66757-1-PBS targets AURKA as part of a matched antibody pair:

MP50265-1: 66757-2-PBS capture and 66757-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20096

Product name: Recombinant human AURKA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 108-337 aa of BC002499

Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET

种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 aurora kinase A
别名 Aurora A, 1F4B10, AIRK1, ARK-1, AURA
计算分子量 46 kDa
观测分子量 46-52 kDa
GenBank蛋白编号BC002499
基因名称 AURKA
Gene ID (NCBI) 6790
RRIDAB_2882103
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14965
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

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