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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 85421-1-RR

    WB analysis using 85421-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85421-1-RR (AUH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85421-1-RR

    Affinity and Kinetic Characterization of 85421-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85421-1-RR against Human AUH were performed. The affinity constant is 29.3 nM.

AUH Recombinant antibody

AUH Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85421-1-RR

产品说明书

CloneNo. 242854F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

242854F12, 3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue, human kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85421-1-RR targets AUH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AUH fusion protein Ag11265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AU RNA binding protein/enoyl-Coenzyme A hydratase
别名 242854F12, 3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18
计算分子量 310 aa, 33 kDa
观测分子量 32 kDa
GenBank蛋白编号BC020722
基因名称 AUH
Gene ID (NCBI) 549
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13825
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AUH antibody 85421-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols