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验证数据展示

  • WB analysis of human brain using 17079-1-AP

    WB analysis of human brain using 17079-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17079-1-AP (AUH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17079-1-AP

    IHC staining of human brain using 17079-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17079-1-AP (AUH antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17079-1-AP

    IHC staining of human brain using 17079-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17079-1-AP (AUH antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HeLa using 17079-1-AP

    IP experiment of HeLa using 17079-1-AP

    IP result of anti-AUH (IP:17079-1-AP, 4ug; Detection:17079-1-AP 1:300) with HeLa cells lysate 2800 ug.

AUH Polyclonal antibody

AUH Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17079-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

AUH

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

RIPSee 1 publications below

产品信息

17079-1-AP targets AUH in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用RIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag11265

Product name: Recombinant human AUH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-310 aa of BC020722

Sequence: MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AU RNA binding protein/enoyl-Coenzyme A hydratase
别名 AUH
计算分子量 310 aa, 33 kDa
观测分子量 32 kDa
GenBank蛋白编号BC020722
基因名称 AUH
Gene ID (NCBI) 549
RRIDAB_2061316
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13825
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AUH antibody 17079-1-APDownload protocol
IP protocol for AUH antibody 17079-1-APDownload protocol
WB protocol for AUH antibody 17079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanRIP

Int J Biol Macromol

m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway

Authors - Aini Pan
View Article
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