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AUH Polyclonal antibody

AUH Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17079-1-AP

Print datasheet

Synonyms

AUH

验证数据展示

  • WB analysis of human brain using 17079-1-AP

    WB analysis of human brain using 17079-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17079-1-AP (AUH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17079-1-AP

    IHC staining of human brain using 17079-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17079-1-AP (AUH antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17079-1-AP

    IHC staining of human brain using 17079-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17079-1-AP (AUH antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HeLa using 17079-1-AP

    IP experiment of HeLa using 17079-1-AP

    IP result of anti-AUH (IP:17079-1-AP, 4ug; Detection:17079-1-AP 1:300) with HeLa cells lysate 2800 ug.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

RIPSee 1 publications below

产品信息

17079-1-AP targets AUH in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsRIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AUH fusion protein Ag11265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name AU RNA binding protein/enoyl-Coenzyme A hydratase
Synonyms AUH
Calculated Molecular Weight 310 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC020722
Gene Symbol AUH
Gene ID (NCBI) 549
RRIDAB_2061316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13825
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AUH antibody 17079-1-APDownload protocol
IHC protocol for AUH antibody 17079-1-APDownload protocol
IP protocol for AUH antibody 17079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanRIP

Int J Biol Macromol

m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway

Authors - Aini Pan
View Article