验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
15693-1-AP targets ATXN10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8259 Product name: Recombinant human ATXN10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-475 aa of BC007508 Sequence: ELRVEQESLLTAFRCGLQFLGNIASRNEDSQSIVWVHAFPELFLSCLNHPDKKIVAYSSMILFTSLNHERMKELEENLNIAIDVIDAYQKHPESEWPFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDIPVFLRHAELIASTFVDQCKTVLKLASEEPPDDEEALATIRLLDVLCEMTVNTELLGYLQVFPGLLERVIDLLRVIHVAGKETTNIFSNCGCVRAEGDISNVANGFKSHLIRLIGNLCYKNKDNQDKVNELDGIPLILDNCNISDSNPFLTQWVIYAIRNLTEDNSQNQDLIAKMEEQGLADASLLKKVGFEVEKKGEKLILKSTRDTPKP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ataxin 10 |
| 别名 | SCA10, E46L, Ataxin-10, ataxin 10 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC007508 |
| 基因名称 | ATXN10 |
| Gene ID (NCBI) | 25814 |
| RRID | AB_2061167 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBB4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| IHC protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| IP protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| WB protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways. | ||
bioRxiv Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties | ||
J Neurochem Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms. |
