验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27265-1-AP targets ATXN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ATXN1 fusion protein Ag26099 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ataxin 1 |
| 别名 | ataxin 1, ataxin1, Ataxin-1, ATX1, ATXN 1 |
| 计算分子量 | 87 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | NM_000332 |
| 基因名称 | ATXN1 |
| Gene ID (NCBI) | 6310 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54253 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ataxin-1 (ATXN1) is a protein involved in transcriptional regulation and RNA binding. It is a transcriptional repressor that interacts with multiple transcription factors and is associated with large protein complexes. ATXN1 is also involved in the regulation of alternative splicing, as demonstrated by its ability to regulate the splicing of several minigenes. The protein is crucial for normal cerebellar function and is implicated in the pathogenesis of spinocerebellar ataxia type 1 (SCA1), a neurodegenerative disorder caused by the expansion of a polyglutamine tract in ATXN1 (PMID: 21475249; 20628574).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATXN1 antibody 27265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |