验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25548-1-AP targets ATRAID in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22168 Product name: Recombinant human C2orf28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC035850 Sequence: MLHARCCLNQKGTILGLDLQNCSLEDPGPNFHQAHTTVIIDLQANPLKGDLANTFRGFTQLQTLILPQHVNCPGGINAWNTITSYIDNQICQGQKNLCNNTGDPEMCPENGSCVPDGPGLLQCVCADGFHGYKCMRQGSF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 2 open reading frame 28 |
| 别名 | Apoptosis related protein 3, APR 3, APR 3, APR3, C2orf28, HSPC013, p18, PRO240, UNQ214/PRO240 |
| 计算分子量 | 229 aa, 25 kDa |
| 观测分子量 | 28-35 kDa |
| GenBank蛋白编号 | BC035850 |
| 基因名称 | ATRAID |
| Gene ID (NCBI) | 51374 |
| RRID | AB_2880128 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UW56 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATRAID, also named as APR3, p18, C2orf28, promotes osteoblast cell differentiation and terminal mineralization. It plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway. This antibody recognizes all the three isoforms (20-24 kDa, 16-19 kDa and 28-30 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATRAID antibody 25548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
