验证数据展示
经过测试的应用
| Positive WB detected in | pig skeletal muscle tissue, human skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88342-1-RR targets CARNS1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag22761 Product name: Recombinant human ATPGD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 443-827 aa of BC036557 Sequence: MTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAALRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATP-grasp domain containing 1 |
| 别名 | ATPGD1, ATP grasp domain containing 1, ATP-grasp domain-containing protein 1, Carnosine synthase 1, EC:6.3.2.11 |
| 计算分子量 | 827 aa, 88 kDa |
| 观测分子量 | 88-103 kDa |
| GenBank蛋白编号 | BC036557 |
| 基因名称 | CARNS1 |
| Gene ID (NCBI) | 57571 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | A5YM72 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CARNS1, located at human chromosome 11q13.2, encodes carnosine synthase 1 belonging to the ATP-grasp ATPase family (EC 6.3.2.11). It mediates ATP-dependent synthesis of carnosine in skeletal muscle and homocarnosine in the central nervous system, two vital histidine-containing dipeptides with antioxidant and pH-buffering effects. This gene is highly conserved across vertebrates and preferentially expressed in brain and muscle tissues. Aberrant CARNS1 expression disturbs dipeptide homeostasis, linking to aging-related sensory decline and tumor progression via impaired carnosine-mediated tumor suppression. (PMID: 20097752)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CARNS1 antibody 88342-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
