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验证数据展示

  • WB analysis using 25725-1-AP

    WB analysis using 25725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25725-1-AP (CARNS1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 25725-1-AP

    IHC staining of mouse liver using 25725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CARNS1 Polyclonal antibody

CARNS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25725-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP grasp domain containing 1, ATPGD1, Carnosine synthase 1, CARNS1, KIAA1394

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25725-1-AP targets CARNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 CARNS1 fusion protein Ag22812 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-grasp domain containing 1
别名 ATP grasp domain containing 1, ATPGD1, Carnosine synthase 1, CARNS1, KIAA1394
计算分子量 827 aa, 88 kDa
观测分子量 88-103 kDa
GenBank蛋白编号BC036557
基因名称 CARNS1
Gene ID (NCBI) 57571
RRIDAB_2880211
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5YM72
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CARNS1 antibody 25725-1-APDownload protocol
IHC protocol for CARNS1 antibody 25725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Part Fibre Toxicol

Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker.

Authors - Chia-Chi Ho
View Article
ratWB

Transl Stroke Res

Nicotine Exposure Along with Oral Contraceptive Treatment in Female Rats Exacerbates Post-cerebral Ischemic Hypoperfusion Potentially via Altered Histamine Metabolism.

Authors - Nathan d'Adesky
View Article
ratWB

Neurochem Res

Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure.

Authors - Krzysztof Milewski
View Article