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验证数据展示

  • WB analysis of NIH/3T3 using 23993-1-AP

    WB analysis of NIH/3T3 using 23993-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23993-1-AP (ATPBD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 23993-1-AP

    IF Staining of NIH/3T3 using 23993-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 23993-1-AP (ATPBD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ATPBD4 Polyclonal antibody

ATPBD4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23993-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

ATP binding domain 4, ATPBD4, Diphthamide synthase, Diphthamide synthetase, Diphthine-ammonia ligase, DPH6, Protein DPH6 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, L02 cells
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23993-1-AP targets ATPBD4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag21178

Product name: Recombinant human ATPBD4 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-259 aa of BC008485

Sequence: GGKDSCYNMMQCIAAGHQIVALANLRPAENQVGSDELDSYMYQTVGHHAIDLYAEAMALPLYRRTIRGRSLDTRQVYTKCEGDEVEDLYELLKLVKEKEEVEGISVGAILSDYQRIRVENVCKRLNLQPLAYLWQRNQEDLLREMISSNIQAMIIKVAALGLDPDKHLGKTLDQMEPYLIELSKKYGVHVCGEGGEYETFTLDCPLFKKKIIVDSSEVVIHSADAFAPVAYLRFLELHLEDKVSSVPDNYRTSNYIYNF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP binding domain 4
别名 ATP binding domain 4, ATPBD4, Diphthamide synthase, Diphthamide synthetase, Diphthine-ammonia ligase, DPH6, Protein DPH6 homolog
计算分子量 267 aa, 30 kDa
观测分子量 48 kDa
GenBank蛋白编号BC008485
基因名称 ATPBD4
Gene ID (NCBI) 89978
RRIDAB_2879392
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L8W6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

实验方案

Product Specific Protocols
WB protocol for ATPBD4 antibody 23993-1-APDownload protocol
IF protocol for ATPBD4 antibody 23993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain Sci

Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.

Authors - Tsung-Ming Hu
View Article