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验证数据展示

  • WB analysis of mouse spleen using 19504-1-AP

    WB analysis of mouse spleen using 19504-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19504-1-AP

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 19504-1-AP

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 19504-1-AP

    IF Staining of HeLa using 19504-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ATP9A Polyclonal antibody

ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19504-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, class II, type 9A
别名 Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA
计算分子量 119 kDa
观测分子量 105 kDa
GenBank蛋白编号NM_006045
基因名称 ATP9A
Gene ID (NCBI) 10079
RRIDAB_10640806
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75110
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

实验方案

Product Specific Protocols
WB protocol for ATP9A antibody 19504-1-APDownload protocol
IHC protocol for ATP9A antibody 19504-1-APDownload protocol
IF protocol for ATP9A antibody 19504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols