验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, 143B cells, HepG2 cells, LNCaP cells, rat heart tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26723-1-AP targets ATP8 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase 8; ATPase subunit 8 |
| 别名 | MT-ATP8 |
| 计算分子量 | 8 kDa |
| 观测分子量 | 8 kDa |
| GenBank蛋白编号 | YP_003024030 |
| 基因名称 | ATP8 |
| Gene ID (NCBI) | 4509 |
| RRID | AB_2880614 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P03928 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP8 antibody 26723-1-AP | Download protocol |
| WB protocol for ATP8 antibody 26723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
Cell Dynamic mapping of proteome trafficking within and between living cells by TransitID | ||
Cell MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels | ||
Cell Metab Aging-induced tRNAGlu-derived fragment impairs glutamate biosynthesis by targeting mitochondrial translation-dependent cristae organization | ||
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing. | ||
Nat Commun MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis |
