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ATP6V1G3 Polyclonal antibody

ATP6V1G3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19523-1-AP

Print datasheet

Synonyms

ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10

验证数据展示

  • WB analysis of HEK-293 using 19523-1-AP

    WB analysis of HEK-293 using 19523-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19523-1-AP

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 19523-1-AP

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse kidney using 19523-1-AP

    IP experiment of mouse kidney using 19523-1-AP

    IP result of anti-ATP6V1G3 (IP:19523-1-AP, 4ug; Detection:19523-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19523-1-AP targets ATP6V1G3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Synonyms ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberNM_133326
Gene Symbol ATP6V1G3
Gene ID (NCBI) 127124
RRIDAB_10642838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1G3,also named as ATP6G3, belongs to the V-ATPase G subunit family. ATP6V1G3 is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

实验方案

Product Specific Protocols
WB protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IHC protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IP protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols