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验证数据展示

  • WB analysis of HEK-293 using 19523-1-AP

    WB analysis of HEK-293 using 19523-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19523-1-AP

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 19523-1-AP

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse kidney using 19523-1-AP

    IP experiment of mouse kidney using 19523-1-AP

    IP result of anti-ATP6V1G3 (IP:19523-1-AP, 4ug; Detection:19523-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

ATP6V1G3 Polyclonal antibody

ATP6V1G3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 19523-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19523-1-AP targets ATP6V1G3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
别名 ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号NM_133326
基因名称 ATP6V1G3
Gene ID (NCBI) 127124
RRIDAB_10642838
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LB4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1G3,also named as ATP6G3, belongs to the V-ATPase G subunit family. ATP6V1G3 is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

实验方案

Product Specific Protocols
IHC protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IP protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
WB protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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