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验证数据展示

  • WB analysis of rat brain using 25316-1-AP

    WB analysis of rat brain using 25316-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25316-1-AP (ATP6V1G2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25316-1-AP

    IHC staining of mouse brain using 25316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25316-1-AP (ATP6V1G2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25316-1-AP

    IHC staining of mouse brain using 25316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25316-1-AP (ATP6V1G2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V1G2 Polyclonal antibody

ATP6V1G2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25316-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

25316-1-AP targets ATP6V1G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag18007

Product name: Recombinant human ATP6V1G2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 40-118 aa of BC119726

Sequence: AQMEVEQYRREREHEFQSKQQAAMGSQGNLSAEVEQATRRQVQGMQSSQQRNRERVLAQLLGMVCDVRPQVHPNYRISA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2
别名 ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10
计算分子量 118 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC119726
基因名称 ATP6V1G2
Gene ID (NCBI) 534
RRIDAB_2880027
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
IHC protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle
View Article
humanWB

EBioMedicine

A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.

Authors - Irene Bertolini
View Article
humanWB

EBioMedicine

Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.

Authors - Andrea Terrasi
View Article