验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
33364-1-AP targets ATP6V0D2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag38739 Product name: Recombinant human ATP6V0D2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-349 aa of BC065207 Sequence: ETLYPTFGKLYPEGLRLLAQAEDFDQMKNVADHYGVYKPLFEAVGGSGGKTLEDVFYEREVQMNVLAFNRQFHYGVFYAYVKLKEQEIRNIVWIAECISQRHRTKINSYIPI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 |
| 别名 | ATP6, ATP6D2, V-ATPase subunit d 2, VMA6, V-type proton ATPase subunit d 2 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC065207 |
| 基因名称 | ATP6V0D2 |
| Gene ID (NCBI) | 245972 |
| RRID | AB_3717331 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N8Y2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP6V0D2 is a subunit of the vacuolar proton-translocating ATPase (V-ATPase) complex, which is responsible for acidifying intracellular compartments and the extracellular environment. This protein is crucial for maintaining the acidic environment necessary for various cellular processes, including bone resorption by osteoclasts (PMID: 19113919). ATP6V0D2 is also involved in the regulation of tumor-associated macrophages, where it influences tumor progression through the lactate/ATP6V0D2/HIF-2α axis (PMID: 30431439).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V0D2 antibody 33364-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
