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验证数据展示

  • WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

ATP6V0D1 Monoclonal antibody, PBS Only

ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68506-1-PBS

产品说明书

CloneNo. 1G7A12

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68506-1-PBS targets ATP6V0D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
免疫原

CatNo: Ag13012

Product name: Recombinant human ATP6V0D1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC008861

Sequence: MSFFPELYFNVDNGYLEGLVRGLKAGVLSQADYLNLVQCETLEDLKLHLQSTDYGNFLANEASPLTVSVIDDRLKEKMVVEFRHMRNHAYEPLASFLDFITYSYMIDNVILLITGTLHQRSIAELVPKCHPLGSFEQMEAVNIAQTPAELYNAILVDTPLAAFFQDCISEQDLDEMNIEIIRNTLYKAYLESFYKFCTLLGGTTADAMCPILEFEADRRAFIITINSFGTELSKEDRAKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEGAGSNPGDKTLEDRFFEHEVKLNKLAFLNQFHFGVFYAFVKLKEQECRNIVWIAECIAQRHRAKIDNYIPIF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
别名 32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD
计算分子量 351 aa, 40 kDa
观测分子量37-41 kDa
GenBank蛋白编号BC008861
基因名称 ATP6V0D1
Gene ID (NCBI) 9114
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP61421
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

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