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验证数据展示

  • WB analysis using 15842-1-AP (same clone as 15842-1-PBS)

    WB analysis using 15842-1-AP (same clone as 15842-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15842-1-AP (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15842-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 15842-1-AP (same clone as 15842-1-PBS)

    IF Staining of MCF-7 using 15842-1-AP (same clone as 15842-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATP5J2 antibody (15842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15842-1-PBS in a different storage buffer formulation.

ATP5J2 Polyclonal antibody, PBS Only

ATP5J2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 15842-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ATP synthase membrane subunit f, ATP5JL, ATP5MF

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15842-1-PBS targets ATP5J2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8598

Product name: Recombinant human ATP5J2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-94 aa of BC003678

Sequence: MASVGECPAPVPVKDKKLLEVKLLELPSWILMRDFSPSGIFGAFQRGYYRYYNKYINVKKGSISGITMVLACYVLFSYSFSYKHLKHERLRKYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
别名 ATP synthase membrane subunit f, ATP5JL, ATP5MF
计算分子量 5-11 kDa
观测分子量 11kDa
GenBank蛋白编号BC003678
基因名称 ATP5J2
Gene ID (NCBI) 9551
RRIDAB_3741870
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56134
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5J2 (also known as ATP synthase-coupling factor 6, or CF6) is a nuclear-encoded subunit of mitochondrial ATP synthase (Complex V), the enzyme responsible for synthesizing ATP from ADP and inorganic phosphate (Pi) during oxidative phosphorylation (OXPHOS).

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