验证数据展示
经过测试的应用
| Positive WB detected in | mouse stomach tissue, rat stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55124-1-AP targets ATP4A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+/K+ exchanging, alpha polypeptide |
| 别名 | ATP4A, ATP6A, Proton pump |
| 计算分子量 | 114 kDa |
| 观测分子量 | 114 kDa |
| GenBank蛋白编号 | NM_000704 |
| 基因名称 | ATP4A |
| Gene ID (NCBI) | 495 |
| RRID | AB_3670239 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20648 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. ATP4A belongs to a family of P-type cation-transporting ATPases, encodes a catalytic alpha subunit of the gastric H+, K+-ATPase, and transports H+ ions in exchange for K+ ions across the apical membrane of parietal cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP4A antibody 55124-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
