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PMCA2 Polyclonal antibody

PMCA2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19678-1-AP

Print datasheet

Synonyms

ATP2B2, PMCA2, PMCA2a, PMCA2i

验证数据展示

  • WB analysis of human brain using 19678-1-AP

    WB analysis of human brain using 19678-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19678-1-AP (PMCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19678-1-AP

    IHC staining of human brain using 19678-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19678-1-AP (PMCA2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 19678-1-AP

    IHC staining of human brain using 19678-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19678-1-AP (PMCA2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

19678-1-AP targets PMCA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 2
Synonyms ATP2B2, PMCA2, PMCA2a, PMCA2i
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 137 kDa
GenBank Accession NumberNM_001001331
Gene Symbol ATP2B2
Gene ID (NCBI) 491
RRIDAB_10644327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. ATG2B2 catalyze the reaction:ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

实验方案

Product Specific Protocols
WB protocol for PMCA2 antibody 19678-1-APDownload protocol
IHC protocol for PMCA2 antibody 19678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors - Jian Xiong
View Article
chickenWB

Metallomics

Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.

Authors - Jie Yang
View Article
WB

Reprod Domest Anim

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.

Authors - M D Xi
View Article
WB

Mol Ther Nucleic Acids

lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.

Authors - Jie Yang
View Article