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验证数据展示

  • WB analysis using 30035-1-AP (same clone as 30035-1-PBS)

    WB analysis using 30035-1-AP (same clone as 30035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30035-1-AP (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30035-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 30035-1-AP (same clone as 30035-1-PBS)

    IHC staining of human colon using 30035-1-AP (same clone as 30035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30035-1-AP (ATP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2B1 antibody included
  • IHC staining of human pancreas cancer using 30035-1-AP (same clone as 30035-1-PBS)

    IHC staining of human pancreas cancer using 30035-1-AP (same clone as 30035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30035-1-AP (ATP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30035-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP2B1 antibody included

ATP2B1 Polyclonal antibody, PBS Only

ATP2B1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30035-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ATP2B1 Ready-To-Use IHC Kit. ATP2B1 primary antibody included.

产品信息

30035-1-PBS targets ATP2B1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32423

Product name: Recombinant human ATP2B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-89 aa of NM_001001323.1

Sequence: GDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Ca++ transporting, plasma membrane 1
别名
计算分子量135 kDa
观测分子量130-180 kDa
GenBank蛋白编号NM_001001323.1
基因名称 ATP2B1
Gene ID (NCBI) 490
RRIDAB_3086214
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20020
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).

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