验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22361-1-AP targets ATP2A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, chicken |
| 免疫原 | ATP2A1 fusion protein Ag17944 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 |
| 别名 | SR Ca(2+)-ATPase 1, SERCA1, EC:7.2.2.10, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, ATP2A 1 |
| 计算分子量 | 1001 aa, 110 kDa |
| 观测分子量 | 116 kDa |
| GenBank蛋白编号 | BC037354 |
| 基因名称 | ATP2A1 |
| Gene ID (NCBI) | 487 |
| RRID | AB_2879089 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O14983 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP2A1 antibody 22361-1-AP | Download protocol |
| IHC protocol for ATP2A1 antibody 22361-1-AP | Download protocol |
| IP protocol for ATP2A1 antibody 22361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice | ||
Metallomics Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken. | ||
Physiol Behav Exposure to melamine cyanuric acid in adult mice caused motor activity and skeletal muscle energy metabolism disorder | ||
J Muscle Res Cell Motil Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes. | ||
Mol Ther Nucleic Acids lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway. |
