ATP1B2 Polyclonal antibody

ATP1B2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22338-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

Adhesion molecule in glia, ATPase Na+/K+ beta 2, Sodium/potassium-dependent ATPase subunit beta-2, Sodium/potassium-transporting ATPase subunit beta-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格价格库存


经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, C6 cells, C2C12 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22338-1-AP targets ATP1B2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 ATP1B2 fusion protein Ag17818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 2 polypeptide
别名 Adhesion molecule in glia, ATPase Na+/K+ beta 2, Sodium/potassium-dependent ATPase subunit beta-2, Sodium/potassium-transporting ATPase subunit beta-2
计算分子量 290 aa, 33 kDa
观测分子量 45-65 kDa
GenBank蛋白编号BC126175
基因名称 ATP1B2
Gene ID (NCBI) 482
RRIDAB_2879077
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14415
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B2 is the β2 subunit of Na+/K+-ATPase which is an essential membrane-bound enzyme responsible for the transport of Na+ and K+ in most eukaryotic cells. ATP1B2 is also called the adhesion molecule on glia (AMOG) and it is highly expressed in normal glia. It is a heavily glycosylated protein that plays a role in cellular adhesion in the CNS. Recently differential expression of ATP1B2 has been found in some glioneuronal tumors (PMID: 23887941, 19371356). This antibody recognizes the endogenous ATP1B2 protein in human brain. The bands between 45 kDa and 65 kDa represent the glycosylated forms of ATP1B2 in different levels (PMID: 8918259).

实验方案

Product Specific Protocols
WB protocol for ATP1B2 antibody 22338-1-APDownload protocol
IHC protocol for ATP1B2 antibody 22338-1-APDownload protocol
IF protocol for ATP1B2 antibody 22338-1-APDownload protocol
IP protocol for ATP1B2 antibody 22338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Mol Gastroenterol Hepatol

Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.

Authors - Yuli Wang
ratWB

Redox Biol

Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration.

Authors - Xiaoying Luo
humanWB

Cancer Cell Int

Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na+/K+-ATPase β2: potential molecular entities to treat invasive brain cancer.

Authors - Natália Barreto
human,ratWB

World J Biol Psychiatry

Altered expression levels of miR-144-3p and ATP1B2 are associated with schizophrenia.

Authors - Bo Pan
humanWB

Eur J Appl Physiol

Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.

Authors - Muath M Altarawneh
ratWB

Neurosci Lett

Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway

Authors - Bo Pan
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