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验证数据展示

  • WB analysis using 84707-5-RR

    WB analysis using 84707-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84707-5-RR

    IHC staining of mouse brain using 84707-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84707-5-RR

    IHC staining of mouse brain using 84707-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 84707-5-RR

    IHC staining of mouse kidney using 84707-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:1000 (under 10x lens).

  • IHC staining of mouse kidney using 84707-5-RR

    IHC staining of mouse kidney using 84707-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:1000 (under 40x lens).

  • Affinity and Kinetic Characterization of 84707-5-RR

    Affinity and Kinetic Characterization of 84707-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

ATP1B1 Recombinant antibody

ATP1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84707-5-RR

产品说明书

CloneNo. 242109D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242109D12, ATP1B 1, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, rat heart tissue, fetal human brain tissue
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84707-5-RR targets ATP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATP1B1 fusion protein Ag7279 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 1 polypeptide
别名 242109D12, ATP1B 1, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit
计算分子量 35 kDa
观测分子量 45-52 kDa
GenBank蛋白编号BC000006
基因名称 ATP1B1
Gene ID (NCBI) 481
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP05026
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

实验方案

Product Specific Protocols
WB protocol for ATP1B1 antibody 84707-5-RRDownload protocol
IHC protocol for ATP1B1 antibody 84707-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols